Marijuana Controversy essays

Marijuana Controversy essays A significant amount of the United States population admits to illegally using marijuana. Many people have found that using medical marijuana, where it is legal, has had nothing but positive results. I have seen many people young and old using marijuana for recreational use in the last four to five years of my life. In my past experiences with marijuana and marijuana users, I have realized that a very large amount of people habitually smoke marijuana. While being around people who use this drug and occasionally using it myself, I have seen both positive and negative effects. Although marijuana may cause health problems and impair a persons judgment, this illegal narcotic may also have many positive effects on economy and our population. The controversy with marijuana all revolves around the proposed legalization of the drug. While there is a large amount of the population who is strongly against the idea, the number of people who are in favor are growing every day. Politicians, mainly democrats and liberals are beginning to take the stoners side. The governor of New Mexico, Gary Johnson, has made it very clear that he is in favor of legalizing the drug, and also that he was once a weed smoker himself (Duhigg, 14). Not only does this raise questions about how our country is currently viewing marijuana, but also how legalizing this drug may completely overturn the prohibition on this billion-dollar industry. The United States government is currently spending almost $40 billion dollars annually on the prevention of drugs in our country (Becker, 32). Once legalized, the government will be able to tax the sales; therefore, making more money to help our struggling and nearly war torn economy. Estimates place the world market value of illegal drugs at several hundred billions of dollars. This puts drugs in the same league as the markets for cigarettes and alcohol (Becker, 33). Our economy would be maki...

Does Content Marketing Help Retain Current Customers

Does Content Marketing Help Retain Current Customers Content marketing is celebrated as an excellent strategy to widen the sales funnel. Producing a variety of content geared toward a prospective customer keeps your brand top of mind, persuading them to move further in the sales process. Rand Fishkin explained it best  when he said that content marketing is at times a long and laborious process of constantly reminding your customers. So its easy to think once that sale is completed, so is the content. This is a huge mistake by marketers- its essentially ignoring and devaluing an individual once they become a customer. Instead there should be a content strategy in place that builds value, strengthens customer trust and increases potential for brand evangelism, upselling and cross-selling opportunities. #ContentMarketing still continues after the sale. It  constantly reminds your customers of you.The After the Close Dilemma After the sale, there is the vital step of keeping your commitments. At its core, it means keeping your customer happy. Did you guarantee your product or service could solve a specific need? Then it had better deliver. In addition to carrying out the agreement, there are natural emotions and hiccups along the way, including: Buyers remorse New influencers and decision makers Resentment or aggression Distrust Failure to build value Ian Lurie of Portent, Inc. describes the post-sale risks perfectly in the following graph: While his example was specific to marketing agencies, every customer goes through a post-sale evaluation of their purchase. Failure on your part to keep commitments and continue to drive home value opens the door for your competition to get the next sale. So what should you be doing to keep the customer? For many businesses, this may mean tailoring content for multiple departments in charge of project delivery, customer service and long-term customer relationship building. Here are recommendations for each of these three key areas, and some examples of companies doing it best. #1 Project Delivery For most business, going back to keeping your commitments falls on the project delivery team. Think of the software, medical or finance industries with a robust sales staff and defined sales process. Often a closed deal is passed to an account manager. And then, unfortunately, a client is often  underwhelmed because the focus is more on the initial sale rather than project delivery. Since most customers have an established and trusted relationship with the sales representative and not the project delivery team, content marketing can help with this sensitive transition between sales and project delivery, helping you  meet a customers expectations and keep them for the long-term. How do you do this? Building Trust With Customers The last thing you want is for your customers to not trust you after youve made the sale. Distrust stems from a variety of circumstances, such as confusion with your service delivery or perhaps a poor experience with your type of business in the past. Content helps lessen any growing  doubt by establishing credibility and initial trust with the prospect. For example, our agency provides our clients with a tip sheet to making web design projects run smoothly. While many of the tips may seem obvious, it positions us as a serious firm that is dedicated to delivering work on time and on budget. It helps customers trust us. Addressing Customer Inquiries How many times have you scrolled through a lengthy FAQ page and never found  an answer? An ideal customer is one that is proactive in educating themselves, so your content marketing should at least  help a customer do that. Free graphic design tool Canva is great at proactively addressing customer questions, taking their help beyond the basic FAQ page. Their DESIGN 101 newsletter guides beginner users on how to create new designs, ultimately drawing them back to the Canva site. While this is a free tool, educating users after their initial sign up keeps the hype alive and more signups coming in. Off the shelf answers like this not only immediately address customer inquiries but save time for the project delivery team. Do more than provide customers with an FAQ. Use email and blog content to help them learn.Does Your Customer Understand The Features? Some of your customers may be purchasing your service or product for the very first time. While the sales process sells the customer on features and expected outcomes, that doesn’t mean that  theyll immediately know how to handle what they just purchased. You will probably have to help them out quite a bit after the sale. For example, You Need A Budget. (YNAB) simplifies their budgeting platform through a library of tutorial videos on how to effectively use their service. Instead of crafting an FAQ and calling it a day, YNAB repurposes many of their customers’ top concerns and questions into video to continue use of the product. If the client does not use the product, they wont see much value in it. Ongoing content ensures continuous usage. Customers purchase based on features and what should happen. They will need help to MAKE it happen.Pointing Out New Needs Upselling is hard, but crucial for service-focused companies  that want to  maintain a viable business. Essentially, you have to be on the lookout to upsell.  A great way to do this is to re-convert existing customers by continuously responding to market demands and creating new product features. For example, when Twitter was rolling out their own advertising platform, Twitter for Business, they partnered with other social media gurus on webinars to educate current Twitter users on social media best practices. By covering new trends in social media, Twitter not only promoted their brand, they upsold Twitter ads. A current customer is still a potential customer. Are you ready to re-convert them to new features?#2 Customer Service In an ideal world, you gathered enough information  in the initial sales process that you’re prepared to react to any client objections, problems or questions. In reality, this isn’t usually  the case. While your established processes, tools, and people have a large impact on the success of customer service, content marketing can also play a role in reducing customer frustration and abandonment. In other words, your content can be that bridge that cross that gap. Addressing Customer Emergencies Customer emergencies are where customer service can either excel or crash and burn. The horror stories of unprofessional and unhelpful customer service departments are shared all over the web. To create a responsive customer service environment, it is important to make sure they have  useful content they can refer to and share with customers. This content might be in the form of informational one-sheets, downloadable help guides, videos or social media that can take some weight off their shoulders when an extraordinary issue arises. A common case study in poor customer service is the airline industry- and the gold standard is Virgin America. Before content marketing was a bonafide strategy, Virgin America’s safety video took a typical safety message  and revamped it for their customers. Delta is doing something similar with their safety videos, too.  I see flight attendants as an interesting mix between safety guards and customer service- this video marries the two in an entertaining way. Solving Common Product Issues Even if you believe your product or service delivery is 100% foolproof, any time  a customer has to handle any part of the execution, chances are theyll run into some issues. Based on how many customers you have, this can become overwhelming for customer service to manage. Similar to creating content internally, many companies are opting to put the content creation in their customers hands. Enabling customers with tools like community forums and knowledge bases is a great way to use content to build customer service (not to mention the SEO benefits). Moz is a perfect example of communities that use  the expertise of their clients to help  new ones. Continuing To Build Trust Remember the importance of building trust during project management? Customer service keeps that foundation secure. Customer mistrust can stem from many occurrences- product recalls, poor media coverage, buggy features, and unsatisfactory delivery–just to name a few. Social media is a perfect tool for a customer service team as long as proper training and processes are in place. Even if you prefer email to handle most of your customers questions, social media can keep you in front of  your followers in a personal way. Dan Norris of WPCurve is an excellent example of continuing to build trust with his potential network. While he lets his service delivery team handle all customer communication, his Twitter followers receive company updates and accomplishments to drive home trust in using WPCurve. Explaining Sensitive Issues Personal concerns and sensitive topics may play out in a business transaction. Its never fun having difficult conversations with a customer, but content can help ease anxieties and keep a project on task. The Vanguard Blog takes the formality and nerves out of personal issues like investment, retirement planning and tax planning through the expertise of Vanguard employees. By publishing blog posts based on common issues and conversations they have with their clients, theyre helping the greater community in handling complex and sensitive issues. #3 Customer Advocacy Within your client base, there are a small percentage of customers who truly love what you do and want to tell people about it. This is a marketers dream, as word of mouth still reigns supreme. Building a customer advocacy base takes a lot of time, but building content that enables your current supporters is a good starting point. Continuing Product Loyalty Once a sale is complete, that doesnt mean your customer is 100% guaranteed to re-purchase when the time comes. Whether it takes days, weeks, months or years for a re-purchase, content can keep your business top of mind when the timing is right. Copyblogger offers membership-only access to third party companies such as New Rainmaker and ongoing discounts to events and training. Their members also receive access to advanced articles, webinars and their community forum for paid members. Continuously providing value after a membership is purchased reminds the customer when theyre ready to renew how invaluable their service has been. Even if your business is not a membership-type business, you can still create a membership feel with exclusive content only available to current or past customers.

Aspect Hypothesis in Relation to Languages Essay

Aspect Hypothesis in Relation to Languages - Essay Example In addition to this, the types and sub types of aspect hypothesis will be established and for each, several examples of inherent, as well as, grammatical aspects in English and Arabic will be provided. A further explanation will be offered to clarify on why the chosen examples, in addition to, how these examples relate to each other.Considering the literal Arabic (Ø §Ã™â€žÃ™ Ã˜ µÃ˜ ­Ã™â€°, al-Fusha), the research of the findings of the research conducted by Barber indicates that the verb is constituted by two aspect tenses (532). These constituents include both the perfective as well as the imperfective. However, there are quite a number of controversial disagreements among the grammarians and, as such, they do not agree as to whether a distinction should be viewed as distinction in aspect or tense or even both. In English, what is considered as the past verb ((Ù Ã˜ ¹Ã™â€ž Ù…Ø §Ã˜ ¶Ã™Å , fi'l maadiy) is used to denote a particular verb (Ø ­Ã˜ ¯Ã˜ «, hadath), which was comple ted a long time ago or even in the near past (Barber 536). However, it indicates nothing regarding the relation of this event that took place in the past to the present status. One of the best examples to illustrate this is the phrase† he arrived† (ÙˆØ µÃ™â€ž", wasala). The phrase was a clear indication of the arrival occurring in the past (Ayres 29). However, it does not provide any sort of information regarding the present status of the person who has arrived (Altman 589). It may be that the person stayed around or he turned and left. In addition to this, the phrase does not indicate.

ADVOCACY LETTER TO DEPARTMENT OF EDUCATION Essay

ADVOCACY LETTER TO DEPARTMENT OF EDUCATION - Essay Example Students with special needs or those who are mentally/ physically retarded, require special attention. Their care and concern should be kept at the highest priority. Expecting these children to study side by side normal children who do not require any special needs shatters their confidence. In such environments normal children often mock about the children with special needs. As stated in ‘Educational Needs’ (n.d.), the specific issues that may affect a special child’s learning are: In spite the fact the children with special needs are often far more intelligent than normal children, it is just a particular manner in which they need to be dealt with in order to prove their mettle and enable their skills to nurture and prosper. If the learning needs of these children are properly addressed, their level of literacy, self- help skills, mobility, confidence and orientation can all be polished to mature levels. This further on, enables these citizens to become contributing and productive citizens of the society. The Solution to the above mentioned grave concerns is the devising of a plan that would be in the best interest of the student in the educational process. Special education services can be formulated that suit every individual child’s particular needs. A Special Education Advocate can be assigned some like natured special children. A Special Education Advocate’s primary responsibilities are the caring of the basic rights of the children with special needs. Once kids are assigned with a Special Education Advocate it becomes easier for schools to organize the special children. A Good Special Education Advocate is well versed in the legal issues pertaining to the rights of a special child. This assists each person connected to the child to be able to realize the laws regarding the handling of the child. These advocated have the ability to search specific legal issues that may be central to a child’s particular case

Travel Let People Be More Understanding Essay Example for Free

Travel Let People Be More Understanding Essay Traveling is an excellent opportunity to go beyond the boundaries of own country. International tourism enables to open new corners of the world and get familiar with unique unknown cultures, which are multiple on the Universe. Yet, what is more important, traveling allows people not to just enlarge the physical limits of regular existence, but it also widens the outlook and even shapes the existing values by showing how others live and perceive the world.   Ã‚   There exist different types of international tourism – leisure tourism, business travel, medical travel, and historical travel. Leisure travel allows people to go to different usually exotic places (Africa, South East Asia etc.) and have a rest there enjoying the seaside, the sun, beautiful beaches. Business travel supposes that a person visits other countries on business trips. Nowadays, medical traveling is becoming more and more popular. People go abroad to cure from certain diseases or simply enhance there health. Finally, the main purpose of historical tourism is to see the historical heritage of the country. Such a trip supposes multiple excursions to different places of interest of the particular country. However, no matter what is the main purpose of traveling abroad, visiting other country opens new boundaries before the person, which reflect in the different things.   Ã‚   First of all, trip abroad enables people to get familiar with new things – geography, culture, history of the visiting place. This upraises the educational level of the person and enhances self-consciousness of him. For example, we all know that Egypt is famous for its pyramids that are thousands years old. Those pyramids are the foremost glory and distinction of the country. However, not all knows how much effort and human lives were put into those constructions. Only seeing those giants own self, it is possible to understand the real value of human life. Seeing those pyramids, one can realize that his life is nothing compared to thousands year old stone. Such important revelation very often makes people reflect on what they do in their life and how much time they have on this world to do something valuable unless they go away. Another relevant example is Turkey. We used to think that Turkey is a great resort with its wonderful beaches and sea. Yet, on coming to Turkey one gets a chance to see exciting historical places, cause Turkey is the place where there are traces of all three major religions – Islam, Christianity, and Judaism. Thus, we can see that international tourism helps to raise educational level and the level of self-consciousness of a person.   Ã‚   Secondly, traveling enables to form own objective point of view independent of those, which are based on TV programs, journal and newspaper articles, radio broadcasts etc. For instance, Pico Iyer (2002) assures that Arab population is not as evil as American officials portray it. All the media has been stating and convincing other countries that Arabs are terrorist and Americans have to fear them. Moreover, special campaign was even launch to assure that the war against terrorist was the only mean to save and help the nations where this terrorism originated. Yet, as author tells, he was greatly surprised visiting   Bolivia, Vietnam, India and other countries to hear that the those nations are actually against American’s decision to initiate the war. Being â€Å"locked† within the borders of one territory, it is impossible to get a clear picture and understand how others perceive you nation and your country, cause mass media might interpret the events and the opinions according to its own benefit. Thus, only visiting other countries, it is possible to get an objective panorama of everything that is going on in the world. Giving the example of Iyer, international traveling enables Americans to tell other nations that USA is not an evil.   Ã‚   To the third, traveling enables to get rid of certain prejudices that might exist in society in the form of â€Å"common opinion†. Again, as Iyer (2002) states, when he took his mother to Syria, she was very pleasantly surprised to realize that this country was much friendlier, much cleaner and more well-off that she previously thought of. It happens very often when we derive our opinion from certain common sources and form a stereotype and prejudice concerning the thing. Thus, traveling destroys stereotypes and creates more space for us to let in other opinions, views, thoughts. Ultimately, it helps us to be more receptive, more attentive and more tolerant to other cultures, to other opinions, which can be different from ours.   Ã‚   Finally, traveling influences the behavior of people. When one goes to China or Japan and gets exposed to this unique special culture, get to know the beliefs and habits of those people, there is always a temptation to follow the example. Watching the very ascetic Japanese man who can sleep on the mattress and eat only seafood, the thought about the excessive exuberance and its unimportance arises itself. Thus, the habit of living luxuriously might change into a habit of living according to the need. When observing how brave and enduring the peoples of Latin America are, the desire of being like them, fighting to the last comes instantly. Thus, traveling is able to change the whole behavior of a person.   Ã‚   To   conclude, it is important to say that traveling doesn’t simply allow to go beyond the border of the country of residence, but it also allows to go far beyond the limits of traditional thinking and mind. Exposing one to different cultures, vies, beliefs, traveling changes people’s values, behavior, destroy stereotypes and widens general outlook. The summarized benefits that traveling gives are the following: Deeper and wider outlook – new knowledge, new insights; Possibility to form personal objective rather than subjective opinion concerning different things based on own experience; Destruction of prejudices and ability to become more flexible and receptive; Gain of new patterns of behavior, new personal qualities.       References Butler R. â€Å"Contemporary Issues in Tourism Development† Routledge, London, 1999- Cooper C. â€Å"Tourism at the Age of Globalization† Routledge, London, 2001 Pico Iyer â€Å"The necessity of Travel† 2002. Jan.27, 2007 http://www.tourismofindia.com/misc/newstravelnecessity.htm

Comparing Hurricane Hits England and Blessing :: English Literature

Comparing Hurricane Hits England and Blessing The narrator in Hurricane Hits England is Grace Nichols who was born in the Carrribean and the narrator in Blessing is perhaps a person in a country suffering drought, probably in Central Africa. I know this by the way that she describes the conditions â€Å"‘silver’ crashes to the ground† and â€Å"the sudden rush of fortune† this suggests that water is given value (silver) And she or her friends or family must know and may have experienced these conditions earlier in her life. Hurricane Hits England has seven stanzas of variously numbered lines. They are very short therefore snappy and add impact to the piece. It keeps you drawn to the poem as it does not have long lines which sometimes have less meaning than these short lines in the poem. Blessing has four stanzas of medium length lines. The poem is laid out so that it carries on flowing; like water. Lots of devices attract you to the poem including: sibilance, alteration, personification, metaphors and onomatopoeia. These can appeal to the reader’s five senses and add impact to keep the reader interested in the piece. There are lots of rhetorical questions in Hurricane Hits England, as if the narrator is asking or pleading to Huracan, Oya, Hattie and Shango (Gods of wind, thunder. Hattie: A famous Caribbean hurricane. The language in both poems is very different; Blessing uses lots of devices and techniques to add imagery to the poem. Such as onomatopoeia: â€Å"The small splash† â€Å"Imagine the drip of it† â€Å"Silver crashes to the ground.† Blessing also uses similes â€Å"The skin cracks like a pod† personification â€Å"as the blessing sings† and a few times alliteration â€Å"flow has found† â€Å"sometimes, the sudden rush† â€Å"polished to perfection.† A metaphor is used in the middle of the poem â€Å"silver crashes to the ground† this is a good line as it also includes onomatopoeia. There is also sibilance â€Å"small splash† and some ambiguous lines. There are a few cases of enjambment and lots of cases of imagery in both poems. However, Hurricane Hits England uses a different approach, using carefully crafted pairs of adjectives to create lots of imagery and appeal to the reader’s senses. â€Å"howling ship† â€Å"gathering rage† There is lots of strong adjectives â€Å"reaping, ancestral, crusted, craving† There is also a simile â€Å"falling heavy as whales† Lots of rhetorical questions are asked â€Å"Tell me why you visit an English coast?† â€Å"Even as you short circuit us into further darkness?† What is the meaning of reaping havoc in new places?† These make you wonder who the narrator is talking to. She talks to Gods in the poem

Red Badge of Courage Essay

â€Å"The central thrust of the Red Badge of Courage is directed towards the puncturing of Henry Fleming’s youthful range of illusions. † â€Å"Our inescapable conclusion concerning Red Badge of Courage is that Fleming is as deluded as the novel ends as he is when he first joins the Union Forces. † Which of the above assessments of the development of Henry Fleming’s character do you feel comes closest to the truth? In your answer you should Consider the arguments for and against accepting the question’s assertions; Bring to bear knowledge of external critical opinion on the issue; Look at contextual aspects of the novel in relation to the topic under consideration. ANSWER I feel that both these arguments show strong opinions and though both show a critical view of Henry Fleming, they are opposing views. The first statement seems to come closest to the truth in that the narrator uses irony to mock and deride Henry yet at the same time he feels a certain affinity with him also. Statement two is a conclusion but not inescapable as Henry, although still deluded, is not as deluded as he was at the time he joined the Union Army. In choosing statement one to be the truer of the two I have come to the conclusion that the narrator is being used as a figure who mocks Henry’s egotism and self deception on one hand then shows sympathy on the other. Stephen Crane uses the narrator to tell the story from a third party point of view and therefore can use this to get his points across. This use of third parties belies Crane’s own experiences of death and mutilation both in his private life with the death of his siblings and in the stories, pictures and photographs he has seen whilst researching the book. It is possible, Stephen Crane had read General Ulysses S Grant’s memoirs and also â€Å"Battles and Leaders of the Civil War†, which was a very popular and factual compendium of four books at the time and he no doubt saw Mathew B Brady’s photographs of the Civil War in these also. The novel is a naturalistic human- interest story but Crane uses the experiences of ordinary soldiers who fought during the Civil War to get the feel of how young men were forever changed by their experiences. Crane cleverly uses contrasts to show how Henry feels at differing times, using monster images to show how active an imagination Henry has, for example to describe a column of men, â€Å"two serpents crawling from the cavern of night†. Crane also uses nature and colour to show contrasts, with constant references to how the sky looks and the fact that â€Å"Mother Nature† still goes on regardless of anything that puny men can do to themselves, for example â€Å"a river, amber tinted in the shadow of its banks, purled at the army’s feet, and at night, when the stream had become of a sorrowful blackness, one could see across it the red, eye-like gleam of hostile campfires†¦ † Phrases like â€Å"A fair field holding life. †¦ It would die if its timid eyes were compelled to see blood† and â€Å"He conceived nature to be a woman with a deep aversion to tragedy† all show Henry’s wonderment at the fact that nature can carry on despite what happens, and at times the narrator sometimes unfairly mocks Henry for this. As the story develops and we see how Henry deals with the harsh realities of war the use of symbols becomes more developed, with Henry seeing the darker sides to war and death. This is when, I feel, the narrator becomes sympathetic to Henry as he tries to reconcile himself with the terrible things he has seen and done. The narrator give the most sympathy to Henry when he gets lost in the woods and finds a dead man, â€Å"horror-stricken by the sight of a thing† and again when he meets up with Jim Conklin and watches as he dies. Paradoxically he mocks when Henry’s thoughts turn to death and the hope that he â€Å"would be understood† in the afterlife. When Henry celebrates victory too soon and then runs away and when Henry leaves the tattered man to die despite staying with Jim whilst he died. After Henry returns to his own camp and Wilson tends his wound he gets very defensive if anyone mentions his absence or his wound, to the point of him thinking of blackmailing Wilson with the return of his letters and the narrator here shows how Henry is feeling superior yet benevolent, thinking himself the better for not being able to conjure up a scathing remark and how his self justification makes him pompous, devious and condescending. Henrys illusions are punctured again when after bravely fighting, he hears the veterans laughing at him, which only provokes him to further prove his own worth on the battlefield. His perception of the battle is now coloured by the â€Å"brotherhood of battle. † The second claim, is wrong in that Henry is not as deluded as he was, he has fought his demons and come out on the other side, not perfect, but able to realise that he has done dreadful things and he will have to live with them. Crane uses this discovery of self to great aplomb as he makes Henry question himself in the final chapter. Henry has a totally selfish and biased view of the world at the start, which slowly unravels as the story goes on. Crane uses the narrator to give us an insight into Henry’s mind by verbalising his thoughts and giving the narrators view of his actions. This in turn helps us to see the turmoil that Henry faces both within fighting the war and within himself. He turns out to despise his early blusterings and convinces himself that he has matured fully. Although this is not true, he has matured to some extent by being able to see the flaws in his character. Henry still romanticises himself and his surroundings but his personal battle between his consious fear and his desire to become a hero has been won. When Henry joined up he believed that he would become a great hero and that he would win great battles, he of course had never even seen a battle except those that went on in his head. He boasts and brags, to himself, about how brave he is going to be yet he turns and runs once the realities of the fighting become apparent. Through his experiences he slowly comes to terms with the fact that war is bloody and cruel And when his friend shows weakness he stores the information to use at a later date – something he later cannot do. To justify all that he has done he thinks † it had been necessary for him to swallow swords that he might have a better throat for grapes. Fate had, in truth been kind to him. † And to justify leaving the tattered man â€Å"he exclaimed that its importance in the aftertime would be great to him if it even succeeded in hindering the workings of his egotism. † Critics of Stephen Crane have both blasted this story as utter rubbish and Crane’s usage of the third party narrator as him trying to write a biography. General McClurg, when this book was published blasted it as blatant lies. His soldiers would never act like that in such a base manner. McClurg himself fought in the Civil War but being a general was not of course in the front lines of the battle. Many of the men who were, swore that they had fought with Stephen Crane even though he was not born until after the Civil War had ended. Such was the reality of the story to those people. The people who thought that this story was an analogy for Stephen Crane’s life based this assumption on these claims, so therefore, Stephen Crane must know of the battle mindset. Personally I think that Crane had heard so many stories of the war he could picture himself there and could imagine how a young boy would feel going through these experiences. Rather like modern stories penned by writers for television and films. I believe that Crane has shown himself to be a psychological realist in writing this piece and that each reader takes from it what they want in relation to their own experiences and knowledge. Perhaps this was a biographical piece and Crane used Henry to show his own delusions. On discussion of this piece, both within a classroom setting and using discussion groups on computer, I have read and heard many differing views of Henry. These views vary depending on the critic’s age. Many young people see him as egotistical, judgemental and self absorbed whilst mature students see him as just like most teenagers, too young to be able to see the big picture. Henry may be deluded but like most people once maturity sets in delusions become lessened as experience is gained. In conclusion I feel that statement 1 is true because the story develops many internal storylines one of which is the puncturing of Henry Fleming’s youthful range of illusions, of which he has many. Statement 2 on the other hand seems true because Henry is deluded to a certain extent. This is one definite statement with no real detail behind it, the author of it seems to be assuming that Henry can not or will not change.

Chromatography for Protein Purification

Department of Chemical & Biomolecular Engineering THE NATIONAL UNIVERSITY of SINGAPORE Chemical Engineering Process Laboratory II Experiment B2 Chromatography for Protein Purification Name Matric No. Group : : : Date of Expt. : GRADE : A. Learning objectives 1. 2. 3. 4. Establish chromatographic assay to determine protein concentrations in a mixture. Appreciate the importance of resolution in protein chromatography. Understand the tension between purity and yield in protein chromatography. Understand the importance of mass balance closure in protein purification.B. Introduction I. Fast Protein Liquid Chromatography (FPLC) High Performance Liquid Chromatography (HPLC) is the workhorse for any biopharmaceutical protein downstream processing train, featuring at least twice within the train. You must recall experiencing the HPLC in one of the experiments in your CN2108 module. Read up on the essential parts of the HPLC system. In this experiment, you will use a modification of the HPLC, the FPLC (Fast Protein Liquid Chromatography System) to separate and purify a mixture of two proteins.The FPLC has been developed to specifically take advantage of the resolution capability of the HPLC for protein purification and collection. II. Concepts in LC When a mixture of proteins is injected into an LC column, the proteins interact with the stationary phase based on their respective chemistries and move through the column at different speed. Based on this differential migration, the proteins elute from the end of the column at different times and therefore become separated. This process is usually facilitated by following the proteins with a mobile phase.Although the protein mixture will have entered as a narrow, concentrated peak, it will exit dispersed and diluted by the mobile phase. This is called bandspreading. Bandspreading (which is an inverse indication of the column efficiency) must be minimized especially for large-scale protein purification. When bandspreading is severe, the proteins may not be sufficiently resolved within a reasonable time-frame. The degree of separation of one component from another is referred to as the resolution (RS), determined based on equation 1 (refer to Fig. 1): RS = VB ? V A 0. (W A + WB ) †¦Eqn. (1) Injection wA VA VB wB Figure 1. Typical protein chromatogram Note that resolution can also be defined based on retention times, instead of volumes. There are various ways to improve resolution, the most straightforward of which is to vary the mode of elution – isocratic versus gradient. Both of these modes are based on the strength of the mobile phase, which directly affects the interactions between the proteins and the stationary phase. In protein chromatography, it is desirable to have high yield as well as high purity of the collected fraction.Yield is the amount of a protein collected as a fraction of the total amount of the same protein fed, while purity is a measure of how much of that protein is in the fraction collected. C. Experimental I. Protein Quantification You will design an experiment to obtain the calibration curves for the two proteins provided using FPLC. You are provided with the following for this experiment: 1. An FPLC system which has been properly set up and equilibrated. You only need to inject 100  µL of each of your samples, and your data will be recorded and analysed by the computer.Note the profile of the mobile phase programmed. 2. A protein mixture containing two proteins (S1 and S2) at concentrations of 1. 0 mg/mL each. II. Protein Purification and Collection You are to perform a chromatographic purification of 1mL of the protein mixture provided. You can expect the chromatogram shown in Fig. 2. Myoglobin Lysozyme Figure 2. Chromatogram of two proteins from FPLC Based on Figure 2, you are to conduct the following: 1. Collect one fraction of the highest yield that is 100% pure S1, and the balance in another fraction. 2.Collect one fraction containing a s much of S1 fed as possible. D. Discussion 1. 2. Briefly describe the experiment that you designed in CI. Explain your choice of the collection times for each of your collection in experiment CII. Determine the yield and purity of each of your collected fractions. Perform a material closure for each of CII (1) and CII (2). Based on your results in (2) above, rationalize the importance of resolution in chromatographic separations. Given the tension between yield and purity, which, in your opinion, is more crucial, yield or purity?How do you propose to improve the resolution of S1 and S2 in this chromatographic purification? 3. 4. Useful Notes 1. Reagents: a. Mixture of two proteins b. Mobile phase – 2M ammonium sulfate in 100mM Phosphate buffer pH 7. 0 c. Elution – 100mM Phosphate buffer pH 7. 0 FPLC to be set up with the appropriate parameters: a. detection wavelength at 280nm b. sample loop – 100  µL c. HIC column for protein separation. d. mobile phase â₠¬â€œ 2 M ammonium sulfate e. mobile phase flow rate: 1 mL/min f. gradient elution – linear gradient 100% to 0% over 10 column volume. 2.

Free Essays on Solar Storms

Solar Stoms Angel has returned to Adams’s Rib to find her roots. She is a 17-year-old girl in search of her family and most of all, who was her mother Hannah Wing (p 21). This is also a spiritual quest for Angel, yet that is only an observation that I became aware of after reading this novel Solar Storms. This novel begins with Angel’s arrival at Adam’s Rib on Tinselman’s Ferry (p21). We are given a small history of the settlers – the British, Norwegians and Swedes. Here the groundwork is being laid out by Linda Hogan on how these lands were shaped, and the importance of the water. We are told of a woman in a canoe appearing out of the fog (p22). Also we are given great detail of the terrain. Angel is on a quest to meet her family and find out who her mother Hannah Wing was. Angel comes to Adam’s Rib at the invitation of her great grandmother, Agnes Irons (p 23). At this point and throughout the novel, Linda Hogan takes great care to show the reader great details of the surroundings so that the reader feels present in the story. Or one with the characters, you can almost smell the fish and tress, see the sky and experience the desolation and barrenness of the land as well as the beauty. We see through this description the simple life people are leading. These people who live off the land. Angel arrives scared, alone with her worldly possessions – two garbage bags (p23). Angel is welcomed by her great grandmother Agnes, who is so happy to see Angel, and caught with great emotion and cries tears of joy at Angel’s arrival (p25). It reminded me of the story of the Prodigal Son from the Bible, and how the father was so filled with joy at his son’s return. Next we are introduced to Dora-Rouge, Angel’s Great Great Grandmother. We are integrated to the way of life in this small town and how people in this small new world of Angel is interconnected – John Husk, Frenchie, Bush, and how these people depen... Free Essays on Solar Storms Free Essays on Solar Storms Solar Stoms Angel has returned to Adams’s Rib to find her roots. She is a 17-year-old girl in search of her family and most of all, who was her mother Hannah Wing (p 21). This is also a spiritual quest for Angel, yet that is only an observation that I became aware of after reading this novel Solar Storms. This novel begins with Angel’s arrival at Adam’s Rib on Tinselman’s Ferry (p21). We are given a small history of the settlers – the British, Norwegians and Swedes. Here the groundwork is being laid out by Linda Hogan on how these lands were shaped, and the importance of the water. We are told of a woman in a canoe appearing out of the fog (p22). Also we are given great detail of the terrain. Angel is on a quest to meet her family and find out who her mother Hannah Wing was. Angel comes to Adam’s Rib at the invitation of her great grandmother, Agnes Irons (p 23). At this point and throughout the novel, Linda Hogan takes great care to show the reader great details of the surroundings so that the reader feels present in the story. Or one with the characters, you can almost smell the fish and tress, see the sky and experience the desolation and barrenness of the land as well as the beauty. We see through this description the simple life people are leading. These people who live off the land. Angel arrives scared, alone with her worldly possessions – two garbage bags (p23). Angel is welcomed by her great grandmother Agnes, who is so happy to see Angel, and caught with great emotion and cries tears of joy at Angel’s arrival (p25). It reminded me of the story of the Prodigal Son from the Bible, and how the father was so filled with joy at his son’s return. Next we are introduced to Dora-Rouge, Angel’s Great Great Grandmother. We are integrated to the way of life in this small town and how people in this small new world of Angel is interconnected – John Husk, Frenchie, Bush, and how these people depen...

Definition and Examples of Voice in Phonetics

Definition and Examples of Voice in Phonetics In phonetics  and phonology, voice refers to the speech sounds produced by the vocal folds (also known as the vocal cords). Also known as voicing. Voice quality refers to the characteristic features of an individuals voice.Voice range (or vocal range) refers to the range of frequency or pitch used by a speaker. Etymology From the Latin call. Examples and Observations John Laver[O]ur social interaction through speech depends on much more than solely the linguistic nature of the spoken messages exchanged. The voice is the very emblem of the speaker, indelibly woven into the fabric of speech. In this sense, each of our utterances of spoken language carries not only its own message, but through accent, tone of voice and habitual voice quality it is at the same time an audible declaration of our membership [in] particular social and regional groups, of our individual physical and psychological identity, and of our momentary mood. The Speech Mechanism Beverly CollinsThe overwhelming majority of sounds found in human speech are produced by an egressive pulmonic airstream, i.e. an outgoing stream of air produced by the lungs contracting (partially collapsing inwards) and thus pushing the air contained within them outwards. This airstream then passes through the larynx (known familiarly as the Adams apple) and along a tube of the complex shape formed by the mouth and nose (termed the vocal tract). A variety of muscles interact to produce changes in the configuration of the vocal tract so as to allow parts of the speech organs to come into contact (or near contact) with other parts, i.e. to articulate. Phoneticians term these anatomical bits and pieces the articulatorshence the term for the branch of science known as articulatory phonetics...The vocal folds (also called the vocal cords) vibrate very rapidly when an airstream is allowed to pass between them, producing what is termed voicethat is, a sort of buzz which one can hear and f eel in vowels and in some consonant sounds. Voicing Peter RoachIf the vocal folds vibrate we will hear the sound that we call voicing or phonation. There are many different sorts of voicing that we can producethink of the differences in the quality of your voice between singing, shouting, and speaking quietly, or think of the different voices you might use reading a story to young children in which you have to read out what is said by characters such as giants, fairies, mice or ducks; many of the differences are made with the larynx. We can make changes in the vocal folds themselvesthey can, for example, be made longer or shorter, more tense or more relaxed or be more or less strongly pressed together. The pressure of the air below the vocal folds (the subglottal pressure) can also be varied [in intensity, frequency, and quality]. The Difference Between Voiced and Voiceless Sounds Thomas P. KlammerTo feel the difference between voiced and voiceless sounds for yourself, place your fingers on your Adams apple and produce first the sound of /f/. Sustain that sound for a few seconds. Now quickly switch to the sound of /v/. You should be able to feel very clearly the vibration that accompanies the sound of /v/, which is voiced, in contrast to the absence of such vibration with /f/, which is voiceless. Voicing is the result of moving air causing the vocal folds (or vocal cords) to vibrate within the larynx behind the cartilage of the Adams apple. This vibration, your voice, is what you feel and hear when you sustain the sound of /v/. Resources Collins, Beverley, and Inger M. Mees.  Practical Phonetics and Phonology: a Resource Book for Students. 3rd ed., Routledge, 2013.Klammer, Thomas P., et al.  Analyzing English Grammar. Pearson, 2007.Laver, John.  Principles of Phonetics. Cambridge University Press, 1994.Roach, Peter.  English Phonetics and Phonology: A Practical Course. 4th ed., Cambridge University Press, 2009.

Leeds Bradford Airport Essay Example | Topics and Well Written Essays - 1500 words

Leeds Bradford Airport - Essay Example Primarily, promotion is aimed at informing the clients the existence of a new product or service in the market (Peterson et al, 2010, p.168). It also appeals to the customers that the products and services to be introduced into the market are better that the ones that have been existence. A number of procedures are available that can be used by the organization to help win customers to its new flight services. These include advertisement, sales promotion, public relations, publicity, and personal selling (Harper, 2003, p.108). An effective promotion by the new budget airline will require an integration of these different approaches into the operations of the organization. Advertisement Advertisement refers to providing information to the targeted clients on the availability of new services in the market. It also appeals to the customers by pointing out the desirable qualities of the new services that distinguishes them from other services available in the market. Advertisement can be done through different media. These include print media (newspapers and magazines), television, radio, internet, or public service advertising. In choosing the medium to use for advertisement of services, the company has to understand the medium that is common among the targeted customers (Harper, 2003, p.109). ... About 40% of the funds need to be set aside for advertisement in television. A larger proportion of the targeted customers are adults engaged in some activities during the day. Thus, these advertisements should be aired out in the evenings. Newspapers and magazines are also common and are effective in providing other details like the flight schedules. This medium should receive 20% of the advertisement funds. Many people have access to radio. However, the advertisement through radio is often less reliable since most people listen to radio while they are attending to other things. Drawing their attention to an advertisement may not succeed and it would require that the advertisement be replicated several times in order to e effective. This medium should be allocated 10% of the funds. The internet is increasing its popularity especially among the young adults and middle-aged individuals. It is more likely that the clients who would use the flight services have access to, and uses, inte rnet services. Internet advertisement should take 20% of the advertisement funds. The remaining 10% should be used for other advertisement means like direct mails to potential clients. Sales promotion Sales promotion activities involve providing incentives to the clients of the company to improve on the sales of the services. Sales promotion involves activities like promotional sports competition, sweepstakes, and sales coupons. One promotion initiative that can be used by the organization is to sponsor some annual or seasonal sports activity like soccer tournament for youths below the age of 23 years in the UK. Besides, the company should issue its flight tickets at much lower